Recently, large-scale analyses of recombinant protein expression have revealed sequence features for codon optimization in unprecedented detail 9, 10. On the other hand, the direct evidence of their influences in recombinant protein expression has been shown using a relatively small number of genes (e.g. The association between these sequence features and protein expression levels has been indicated by omics analyses of endogenous genes (e.g. Since stable mRNA secondary structures may inhibit translation, this approach is considered to improve recombinant protein expression by enhancing translational efficiency. Another approach is to introduce synonymous substitution computationally predicted to destabilize mRNA secondary structures. The basis of this approach is that endogenous genes whose coding sequences consist of frequent codons have high protein expression levels, and thus recombinant protein expression is also considered to be improved by increasing the codon frequency. For example, a conventional approach is to substitute rare codons by frequent codons according to the genomic codon usage in a host organism. A key step in recombinant protein expression is codon optimization where a coding sequence for a protein of interest is designed by synonymous substitution aiming to increase its expression level 2.Ĭurrent approaches to codon optimization are based on sequence features considered to influence protein expression levels 3, 4, 5, 6. Recombinant protein expression using bacterial and other host organisms is a fundamental technology for protein production 1. erythropolis, and possibly in other actinobacteria. These results demonstrate the effectiveness of our codon optimization method in R. Especially, for 5 genes whose expression levels for wild-type sequences are small or not detectable, all of them are improved by optimized sequences. We optimize the coding sequences of 12 genes regarding these sequence features, and confirm that 9 of them (75%) achieve increased expression levels compared with wild-type sequences. Intriguingly, other sequence features such as the codon repetition rate show a different tendency from the previous study on E. The statistical analysis of these expression data reveals that the mRNA folding energy at 5’ regions as well as the codon frequency are important sequence features for codon optimization. erythropolis with the same plasmid vector. We evaluate the recombinant protein expression of 204 genes in R. Here, we develop a codon optimization method for Rhodococcus erythropolis, a gram-positive GC-rich actinobacterium attracting attention as an alternative host organism. However, these studies have been limited to common host organisms such as Escherichia coli. Recent studies have investigated sequence features for codon optimization based on large-scale expression analyses. To maximize the chance of success, NovoPro protein expert will design a construct based on the characteristics and specific requirements of the target protein.Codon optimization by synonymous substitution is widely used for recombinant protein expression. Its comprehensive codon optimization algorithm considerate dozens of key factors of gene transcription and translation. The NovoPro’s codon optimization tool - ExpOptimizer is developed for the high expression of any target proteins in any mainstream expression hosts. Proprietary codon optimization tool and professional construct design *Flexible with any customer’s requirements *100% guarantee the amount and purity, no cost if the project fails *Free protein analysis and project design ![]() Partner and explore Novo-frontiers in science.Īll the package services we are providing, are a customized, one-stop and 100% As our name, we aim to accelerate protein research by being a reliable NovoPro offers various recombinant protein & rAb expression platforms for anyĬustom requirements. Proteins, toxic proteins and for target proteins greater than 100kDa). Which, any charge to the customer will be waived (exclusions are applicable to membrane In all cases, final protein will be guaranteed to meet listed specifications, failing.Insoluble proteins maybe subject to protein refolding procedures.Proprietary, ExpOptimizer™ technology, is recommended to be performed during gene synthesis. To increase the expression level of your protein, codon optimization using our.Protein analysis and project proposal is utmost welcome and totally free.*Protein amount is flexible according to the customer’s request. *Free gene synthesis, free subcloning, FREE-If-Fail guarantee Customer Provides Guaranteed Bacterial Protein Expression Services NovoBac TM E.coli Expression, One-stop Solution with Guaranteed Success
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